Selected Publications of ProBioGen


Modeling Human Immunity In Vitro:
Improving Artificial Lymph Node Physiology by Stromal Cells

Sardi M, Lubitz A, Giese C
Applied In Vitro Toxicology. September 2016 2(3): 143-150. doi:10.1089/aivt.2016.0004.

t4 Workshop Report:Biology-Inspired Microphysiological System Approaches to Solve the Prediction Dilemma of Substance Testing

Marx U, Giese C, et al.
Altex - ALTERNATIVES TO ANIMAL EXPERIMENTATION. Issue 03/2016. www.altex.ch/All-issues/Issue.50.html

Continuous cell lines from the muscovy duck as potential replacement for primary cells in the production of avian vaccines

Jordan I, John K, Höwing K, Lohr V, Penzes Z, Gubucz-Sombor E, Fu Y, Gao P,
Harder T, Zádori Z, Sandig V.
Avian Pathol. 2016 Jan 26:1-51. PubMed PMID: 26814192.


Impaired antiviral response of adenovirus-transformed cell lines supports virus

Bachmann M, Breitwieser T, Lipps C, Wirth D, Jordan I, Reichl U, Frensing T.J Gen Virol. 2015 Dec 8. doi: 10.1099/jgv.0.000361. PubMed PMID: 26647282.


CD26/DPP4 cell-surface expression in bat cells correlates with bat cell susceptibility to Middle East respiratory syndrome coronavirus (MERS-CoV) infection and evolution of persistent infection.

Caì Y, Yú SQ, Postnikova EN, Mazur S, Bernbaum JG, Burk R, Zhāng T, Radoshitzky SR, Müller MA, Jordan I, Bollinger L, Hensley LE, Jahrling PB, Kuhn JH
PLos One. 2014 Nov 19; 9(11):e112060. doi: 10.1371/journal.pone.0112060. eCollection 2014; PMID: 25409519

Influenza A Virus Polymerase Is a Site for Adaptive Changes During Experimental Evolution in Bat Cells.

Poole DS, Yú SN, Caì Y, Dinis JM, Müller MA, Jordan I, Friedrich TC, Kuhn JH, Mehle A
J Virol. 2014 Aug 20; pii: JVI.01857-14. [Epub ahead of print] PubMed PMID: 25142579

Boosting Upstream, Downstream Processing: To Expedite Biomanufacturing, Deploy a New Genotype of Modified Vaccinia Virus Ankara.

Lohr V, Sandig V, Jordan I
GEN Genetic Engineering & Biotechnology News, 2014 Jul 1 (Vol. 34, No. 13).

The avian cell line AGE1.CR.pIX characterized by metabolic flux analysis.

Lohr V, Hädicke O, Genzel Y, Jordan I, Büntemeyer H, Klamt S, Reichl U
BMC Biotechnol. 2014 Jul 30; 14(1):72. [Epub ahead of print] PubMed PMID: 25077436.

Propagation of viruses infecting waterfowl on continuous cell lines of Muscovy duck (Cairina moschata) origin.

Mészáros I, Tóth R, Bálint A, Dán A, Jordan I, Zádori Z
Avian Pathol. 2014 Jul 3; 1-28. [Epub ahead of print] PubMed PMID: 24992264.

Matrix and backstage: cellular substrates for viral vaccines.

Jordan I, Sandig V
Viruses. 2014 Apr 11; 6(4):1672-700. doi: 10.3390/v6041672. PubMed PMID: 24732259; PubMed Central PMCID: PMC4014716.

Human immunity in vitro - Solving immunogenicity and more.

Giese C, Marx U
ADDR 2014 Apr; 69-70:103-122

The influence of cell growth and enzyme activity changes on intracellular metabolite dynamics in AGE1.HN.AAT cells.

Rath AG, Rehberg M, Janke R, Genzel, Y, Scholz S, Noll T, Rose T, Sandig V, Reichl U
J Biotechnol. 2014 May 20; 178:43-53. doi: 10.1016/j.jbiotec.2014.03.012. Epub 2014 Mar 18. PMID: 24657347

High cell density cultivations by alternating tangential flow (ATF) perfusion for influenza A virus production using suspension cells.

Genzel Y, Vogel T, Buck J, Behrendt I, Ramirez DV, Schiedner G, Jordan I, Reichl U
Vaccine. 2014 Feb 25; pii: S0264-410X(14)00189-3. doi: 10.1016/j.vaccine.2014.02.016. [Epub ahead of print] PubMed PMID: 24583003


Elements in the Development of a Production Process for Modified Vaccinia Virus Ankara.

Jordan I., Lohr V, Genzel Y, Reichl U, Sandig V
Microorganisms 2013, 1, 100–121.

Continuous influenza virus production in cell culture shows a periodic accumulation of defective interfering particles.

Frensing T, Heldt FS, Pflugmacher A, Behrendt I, Jordan I, Flockerzi D, Genzel Y, Reichl U
PLoS One. 2013 Sep 5; 8(9):e72288. doi: 10.1371/journal.pone.0072288. eCollection 2013. PubMed PMID:  24039749; PubMed Central PMCID: PMC3764112.

A novel genotype of MVA that efficiently replicates in single cell suspensions.

Jordan I., Sandig V
BMC Proceedings 2013, 7(Suppl 6):O1. doi:10.1186/1753-6561-7-S6-O1.

Moderne Herstellprozesse für Impfstoffe.

Jordan I., Lohr V
Transkript 2013, 8-9, 48-50

A genotype of modified vaccinia Ankara (MVA) that facilitates replication in suspension cultures in chemically defined medium.

Jordan I, Horn D, John K, Sandig V
Viruses. 2013 Jan 21; 5(1):321-39. doi: 10.3390/v5010321. PubMed PMID: 23337383; PubMed Central PMCID: PMC3564123


Live attenuated influenza viruses produced in a suspension process with avian AGE1.CR.pIX cells.

Lohr V, Genzel Y, Jordan I., Katinger D, Mahr S, Sandig V, Reichl U
BMC Biotechnol. 2012 Oct 30; 12:79. doi: 10.1186/1472-6750-12-79. PubMed PMID: 23110398; PubMed Central PMCID: PMC3505166.

Production of a Viral-Vectored Vaccine Candidate Against Tuberculosis.

Jordan I., Woods N., Whale G., Sandig V.
BioProcess International; Sep 2012

Fucose-targeted glycoengineering of pharmaceutical cell lines.

Ogorek C., Jordan I., Sandig V., von Horsten H.H.
Methods Mol Biol. 2012; 907:507-17
PMID: 22907371 [PubMed - in process]

Crosstalk between immune cells and mesenchymal stromal cells in a 3D bioreactor system.

Seifert M., Lubitz A., Trommer J., Könnig D., Korus G., Marx U., Volk H. D., Duda G., Kasper G., Lehmann K., Stolk M., Giese C.
Int J Artif Organs 2012; 35: (11) 986-995


N-glycosylation and biological activity of recombinant human alpha1-antitrypsin expressed in a novel human neuronal cell line

Véronique Blanchard, Xi Liu, Susann Eigel, Matthias Kaup, Silke Rieck, Sabina Janciauskiene, Volker Sandig, Uwe Mark, Peter Walden, Rudolf Tauber, Markus Berger
Biotechnology and Bioengineering, Volume 108, Issue 9, pages 2118-2128, September 2011

A chemically defined production process for highly attenuated poxviruses.

Jordan I., Northoff S., Thiele M., Hartmann S., Horn D., Höwing K., Bernhardt H., Oehmke S., von Horsten H., Rebeski D., Hinrichsen L., Zelnik V., Mueller W., Sandig V.
Biologicals. 2011 Jan; 39(1): 50–8. Epub 2011 Jan 15. 
PMID: 21237672 [PubMed – in process] 


Quantitative characterization of metabolism and metabolic shifts during growth of the new human cell line AGE1.HN using time resolved metabolic flux analysis.

Niklas J., Schräder E., Sandig V., Noll T., Heinzle E.
Bioprocess Biosyst Eng. 2010 Dec 25. [Epub ahead of print]
PMID: 21188421 [PubMed - as supplied by publisher] 

Production of non-fucosylated antibodies by co-expression of heterologous GDP-6-deoxy-D-lyxo-4-hexulose reductase. 

von Horsten HH, Ogorek C., Blanchard V., Demmler C., Giese C., Winkler K., Kaup M., Berger M., Jordan I., Sandig V.
Glycobiology. 2010 Dec; 20 (12):1607-18. Epub 2010 Jul 15.
PMID: 20639190 [PubMed - indexed for MEDLINE] 

Prediction of cytokine release using gene expression profile analysis.

Radke L, Lopez D. A., Lubitz A., Giese C., Frohme M.
Chemistry Today. 2010, Vol. 28 n 5, Sept./Oct. 2010,
Focus on Pharmaceutical analysis

Engineering tissue alternatives to animals: applying tissue engineering to basic research and safety testing.

Holmes A., Brown R., Shakesheff K., Giese C.
Regen. Med. 2010, 4(4), 579–592

Establishment of fruit bat cells (Rousettus aegyptiacus) as a model system for the investigation of filoviral infection.

Krähling V., Dolnik O., Kolesnikova L., Schmidt-Chanasit J., Jordan I., Sandig V., Günther S., Becker S.
PLoS Negl Trop Dis. 2010 Aug 24; 4(8): e802.
PMID: 20808767 [PubMed - indexed for MEDLINE] Free PMC Article

Immunological substance testing on human lymphatic micro-organoids in vitro.

Giese C., Lubitz A., Demmler C., Reuschel J., Bergner K., Marx U.
Journal of Biotechnology 2010, Volume 148, Issue 1, 1 July 2010, Pages 38–45


Cell lines from the Egyptian fruit bat are permissive for modified vaccinia Ankara.

Jordan I., Horn D., Oehmke S., Leendertz FH, Sandig V.
Virus Res. 2009 Oct; 145(1): 54-62. Epub 2009 Jun 18.
PMID: 19540275 [PubMed - indexed for MEDLINE] 

New avian suspension cell lines provide production of influenza virus and MVA in serum-free media: studies on growth, metabolism and virus propagation.

Lohr V., Rath A., Genzel Y., Jordan I., Sandig V., Reichl U.
Vaccine. 2009 Aug 6; 27(36): 4975-82. Epub 2009 Jun 14.
PMID: 19531390 [PubMed – indexed for MEDLINE] 

Glycan analysis in cell culture-based influenza vaccine production: influence of host cell line and virus strain on the glycosylation pattern of viral hemagglutinin.

Schwarzer J., Rapp E., Hennig R., Genzel Y., Jordan I., Sandig V., Reichl U.
Vaccine. 2009 Jul 9; 27(32): 4325-36. Epub 2009 May 14.
PMID: 19410619 [PubMed – indexed for MEDLINE] 

Trends in cell culture technology.

Marx, U.
to be published in:New Technologies for Toxicity Testing, Ed.: Michael Balls, 
Landes Bioscience. 


An avian cell line designed for production of highly attenuated viruses.

Jordan I., Vos A., Beilfuss S., Neubert A., Breul S., Sandig V.
Vaccine. 2009, Jan 29; 27(5): 748–56. Epub 2008 Dec 9.
PMID: 19071186 [PubMed – indexed for MEDLINE] 

Quality for Biologics

Zietze, S., Riedel, M.
Biopharm Knowledge Publishing. 2008

Standardization, evaluation and early-phase method validation of an analytical scheme for batch-consistency N-glycosylation analysis of recombinant produced glycoproteins.

Zietze, S., Müller, R. H., Brecht, R.
European Journal of Pharmaceutics and Biopharmaceutics. 2008, 68: 818–827

Azabrendanes V. Synthesis and reactions of stereoisomeric exo- and endo-5-aminomethylbicyclo[2.2.1]hept-2-ene–based ureas.

Lilija I. Kasyan, Andrey O. Kasyan, Igor N. Tarabara, Sergiy I. Okovytyy, Elena A. Golodayeva, Andrey V. Tokar, Yevgeniy Podolyan
Central European Journal of Chemistry. 6(2) 2008: 161–174


An Avian Designer Cell Line for Vaccine Manufacture

Jordan, I., Vos, A., Neubert, A., Sandig, V.
BioWorld Europe. 03–2007

Oligomerization and Transglutaminase Crosslinking of the Cystatin CRES in theMouse Epididymal Lumen: Potential Mechanism of the extracellular Quality Control

von Horsten, H. H., Johnson, S. S., SanFrancisco, S. K., Hastert, M. C., Whelly, S.M., Cornwall, G. A.
Journal of Biological Chemistry. 2007, 282(45): 32912–23

Extracellular quality control in the epidermis

Cornwall, G.A.; von Horsten H.H.; Swartz, D.; Johnson, S.; Chau, K.; Whelly, S.
Asian Journal of Andrology. 2007 Jul, 9(4): 500–7

Physical and biological properties of barium cross-linked alginate membranes

Zimmermann H., Wählisch F., Baier C., Westhoff M., Reuss R., Zimmermann D., Behringer M., Ehrhardt F., Katsen-Globa A., Giese C., Marx U., Sukhorukov V., Vásquez J., Jakob P., Shirley S., Zimmermann U.
Biomaterials 28. 2007, 1327–1345

Novel Bioreactors for Fragile Glycoproteins

Langhammer S., Brecht R., Marx U.
GEN. 2007, January 1: 34–35 

Why Is Human Tissue Culture Important to Drug Discovery?

Marx U.
In: Marx and Sandig: Drug Testing In Vitro – Breakthroughs and Trends in Cell Culture Technology, Wiley-VCH Verlag GmbH & Co. KgaA. 2007. 

Overview on Bioreactor Design, Prototyping and in Process Controls for Reproducible Three Dimensional Tissue Culture. 

Pörtner R., Giese C.
In: Marx and Sandig: Drug Testing In Vitro – Breakthroughs and Trends in Cell Culture Technology, Wiley-VCH Verlag GmbH & Co. KgaA. 2007. 

Development of Human Designer Cell Lines to Enable Sustainable Supply of Differentiated Human Cells.

Sandig V. and Jordan I.
In: Marx and Sandig: Drug Testing In Vitro – Breakthroughs and Trends in Cell Culture Technology,


A Human Lymph Node In Vitro – Challenges and Progress

Giese C., Demmler C.D., Ammer R., Hartmann S., Lubitz A., Miller L., Müller R. and Marx U.
Journal of Artificial Organs. 2006, 30(10):803–808

Three-Dimensional Characterization of Cell Clusters Using Synchroton-Radiation-Based Micro-Computed Tomography
Müller B., Riedel M., Thurner P. J.

Microsc. Microanal. 2006, 12. 97–105 


Alternative Strategies and New Cell Lines for High-level Production of Biopharmaceuticals

Rose, T.; Winkler, K.; Brundke, E.; Jordan, I.; Sandig, V.
In: Knäblein: Modern Biopharmaceuticals, Wiley-VCH. 2005. 761-778

Development and Assessment of Human Adenovirus Type 11 as a Gene Transfer Vector

Stone, D.; Ni, S.; Li, Z:Y:, Gaggar, A.; diPaolo, N., Feng, Q.; Sandig, V.; Lieber, A.
Journal of Virology. 2005. April. 5090-5104

Boosting Mammalian Cell-line Manufacturing Pilot Plant – A Case Report

Brecht, R.; Sandig, V.; Marx, U.; Riedel, M.; Koch., S.
BioPharm International. 2005. Vol. 18. No. 7. 

Mammalian cells

Sandig, V.; Rose, Th.; Winkler, K.; Brecht; R.
In: G. Gelissen: Production of recombinant proteins. Wiley-VCH. 2005. 233-252.


Linker peptide and affinity tag for detection and purification of single-chain Fv fragments

Küttner G., Giessmann E., Wessner H., Scholz C., Reinhardt D., Winkler K., Marx U., Höhne W.
Biotechniques. 2004 May, 36(5):864-870

Genome Size and Structure Determine Efficiency of Postinternalizytion Steps and Gene Transfer of Capsid-Modified Adenovirus Vectors in a Cell-Type-Specific Manner

Shayakhmetov, D.M., Li Z.Y., Gaggar, A., Gharwan, H., Ternovoi, V., Sandig, V., Lieber, A.
Journal of Virology. 2004 Sept, 10009–10022


Intracellular transport of plant toxins ricin and viscumin from different plasma membrane sites

Moisenovich M., Agapov I., Marx U., Bereiter-Hahn J., Tonevitsky A.
Arzneimittelforschung. 2003, 53(6): 470–475

Novel B-ring modified allocolchicinoids of the NCME series: design, synthesis, antimicrotubule activity and cytotoxicity

Bergemann S., Brecht R., Buttner F., Guenard D., Gust R., Seitz G., Stubbs M.T., Thoret S.
Bioorg. Med. Chem. 2003 Apr 3. 11(7):1269–1281

The complete nucleotide sequence, genome organization, and orign of human adenovirus type11

Stone D., Furthmann A., Sandig, V., Lieber, A.
Virology. 2003 Apr 25. 309(1): 152–165


Anti-human CD4 induces peripheral tolerance in a human CD4+, murine CD4-, HLA-DR+ advanced transgenic mouse model

Laub, R.; Brecht, R.; Dorsch, M.; Valey, U.; Wenk, K., Emmrich, F.
J Immunol. 2002 Sep 15. 169 (6): 2947–2955

Comparision between the mechanisms of action of plant toxins ricin and viscumin on the stage of intracellular dissociation

Tonevitsky, A.; Agapov, I.; Chelnokova, O.; Moisenovich, M.; Marx, U.
Arzneimittelforschung. 2002. 52(6): 500–505

Detection of isolated mistletoe lectin chains in plant extracts

Tonevitsky, A.G.; Marx, U.; Agapov, I.; Moisenovich, M. 
Arzneimittelforschung. 2002. 52(1): 67–71


Impact of nanometer-scale roughness on contract-angle hysteresis and globulin adsorption

Müller, B.; Riedel, M.; Michel, R.; dePaul, S.M.; Hofer, R.; Heger, D.; Grützmacher, D.
J. Vac. Sci. Technol. 2001. Sept/Oct. B 19(5)

Protein adsorbtion and monocyte activation on germanium nanopyramides

Riedel, M.; Müller, B.; Wintermantel, E. 
Biomaterials. 2001. 22, 2307–2316

An improved helper-dependent adenoviral vector allows persistent gene expression after intramuscular delivery and overcomes preexisting immunity to adenovirus

Maione, D.; dellaRocco, C.; Gianetti, P.; dÁrrigo, R.; Liberatoscoli, L.; Franlin, LL; Sandig, V.; Ciliberto, G.; laconica, N.; Savino, R.
PNAS. 2001. May 22. vol.98. no.11, 5986–5992

The impact of nanometer – scale roughness on contact – angle hysteresis and globulin adsorption

Müller, B.; Riedel, M.; Michel, R.; De Paul, S.M.; Hofer, R.; Heger, D.;  Grützmacher, D.
J. Vacuum Sci. Technol. 2001. B 19 (5) 1715–1720